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between the inside and outside of the gel particles This can happen if, for example, a solute has a nonzero net charge but is too large to enter the pores, while its small counterions can enter the gel This results in ion-exchange properties in localized areas of the gel These types of electrostatic interactions may be suppressed by increasing the ionic strength of the eluent, which can be accomplished using volatile electrolytes that are readily removed from the separated components following the chromatographic run Adsorption of the solute onto the gel matrix can also occur, but is not normally encountered in SEC It may be counteracted by changing the eluent The ideal eluent is a good solvent for both the solute and the substance forming the gel matrix.

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f P R;

Provided that (N S K ) K , equation (11.6) also bounds the delay for all stripes. To see why, begin with equation (11.3): NR = (k j + 1) K (N S K ) ((N S K ) K ) (11.7)

4:7

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In addition, hydrogen-bond-breaking agents such as urea or guanidinium, or chaotropic ions, or surfactants may be added to eluents to improve SEC characteristics To test for either adsorptive or electrostatic interactions, the SEC separation is performed at a variety of temperatures If the separation occurs by size alone, the retention coef cient R Vo =Ve is independent of temperature; very small variations may be observed as a result of gel swelling or microstructural changes to the gel The presence of a signi cant dependence of R on T indicates the presence of a mechanism other than size exclusion While R should not vary with T, diffusion coef cients increase with T and so zone broadening occurs, leading to decreased resolution with increasing separation temperatures Normally, an approximately linear relationship is observed between Kav and log(MW) over the fractionation range of the gel.

if f P R; otherwise;

(k j + 1) ,

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and, for any A & O, I A inf oPA I f . The function I is a good rate function for the centered Gaussian measure P and the following large deviation principle holds: , for F closed in O: for G open in O: lim sup 1 Z log P p P F I F ; n n3I n 1 Z lim inf log P p P G ! I G : n3I n n

The expanded relationship is, in fact, sigmoidal, as shown in Figure 145 The calibration curve can be linearized using the logit transformation, where logit Kav ln Kav = 1 Kav is plotted against log(MW) Resolution and separations in SEC are described by the van Deemter equation (Eq 147) The main contributions to zone broadening, in order of importance, are as follows The rst factor involves the kinetics of partitioning between the mobile phase and the gel; there is a limited rate at which equilibrium can be established, and this rate depends on the solute s diffusion coef cient Second, differences in the lengths of different stream paths in the packed bed of irregularly shaped particles result in eddy diffusion ; with an ideally packed column, this is not a signi cant problem, but in practice, eddy diffusion may be signi cant Finally, longitudinal.

< (k j + 2) = N F This shows that the delay experienced by stripes {i|i > k + 1} transmitted after the failure is detected (N R ) is smaller than the delay experienced by stripe j (N F ). Therefore, the worst-case delay in equation (11.6) also bounds the delay for all stripes. The additional delay will likely lead to video playback hiccups for the clients. If temporary service interruption can be tolerated, then the clients can simply suspend playback for D F seconds to resynchronize with the new transmission schedule. Otherwise, we can introduce additional buffers at the client to sustain non-stop video playback during recon guration (Section 11.5).

In order to use Theorem 4.4.1 for studying the asymptotics of Q x , we have to make a scaling transformation rst: Q x P sup Zt t > x H P sup x Zt=x t > x H 1 P sup x Zt=x t=x > 1 H 1 P sup x ZT T > 1 P

T!0 t!0 t!0 t!0

Figure 11.5 depicts the server recon guration process for sub-schedule striping, with N S = 5 and K = 1. Instead of considering service rounds, we consider micro-rounds de ned as the period for transmitting a stripe. Hence, a system with N S servers will have N S micro-rounds

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adding barcodes to excel using barcode fonts . ... Follow instructions in Enable Developer Tab in Office 2007/ 2010 to enable the tab first. macro security settings  ...

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